From animal domestication to human genome variation, from loblolly genomes to lager genomes, from wild zebrafish sex to untangled metagenomes, last year brought plenty of high points for the GSA journals. So gathering a small selection of those high points into the 2014 Spotlight booklets was a challenging, but rewarding task for the editors of GENETICS and G3. Each Spotlight is a showcase of the excellent research and scholarship published over the course of the year, along with a selection of striking images submitted by our authors.
Enjoy the 2014 Spotlights at the linked images below. Full text of the articles featured in Spotlight are also available below. You can also browse the 2013 GENETICS Spotlight and the 2013 G3 Spotlight.
GENETICS SPOTLIGHT CITATIONS:
CELLULAR GENETICS
Menzel J., M.E. Malo, C. Chan, M. Prusinkiewicz, T. G. Arnason & T. A. A. Harkness (2014). The Anaphase Promoting Complex Regulates Yeast Lifespan and rDNA Stability by Targeting Fob1 for Degradation, Genetics, 196 (3) 693-709. DOI: 10.1534/genetics.113.158949 http://www.genetics.org/content/196/3/693.full
COMPLEX TRAITS
Li Y., R. Cheng, K. A. Spokas, A. A. Palmer & J. O. Borevitz (2014). Genetic Variation for Life History Sensitivity to Seasonal Warming in Arabidopsis thaliana, Genetics, 196 (2) 569-577. DOI: 10.1534/genetics.113.157628 http://www.genetics.org/content/196/2/569.full
DEVELOPMENTAL & BEHAVIORAL GENETICS
Hoover C.M., S.L. Edwards, S.-c. Yu, M. Kittelmann, J. E. Richmond, S. Eimer, R. M. Yorks & K. G. Miller (2014). A Novel CaM Kinase II Pathway Controls the Location of Neuropeptide Release from Caenorhabditis elegans Motor Neurons, Genetics, 196 (3) 745-765. DOI: 10.1534/genetics.113.158568 http://www.genetics.org/content/196/3/745.full
COMMENTARY
Nurrish S. (2014). Dense Core Vesicle Release: Controlling the Where as Well as the When, Genetics, 196 (3) 601-604. DOI: 10.1534/genetics.113.159905 http://www.genetics.org/content/196/3/601.full
GENE EXPRESSION
Wang G., E. Yang, C. L. Brinkmeyer-Langford & J. J. Cai (2014). Additive, Epistatic, and Environmental Effects Through the Lens of Expression Variability QTL in a Twin Cohort, Genetics, 196 (2) 413-425. DOI: 10.1534/genetics.113.157503 http://www.genetics.org/content/196/2/413.full
GENETICS OF SEX
Wilson C.A., S.K. High, B. M. McCluskey, A. Amores, Y.-l. Yan, T. A. Titus, J. L. Anderson, P. Batzel, M. J. Carvan, M. Schartl & J. H. Postlethwait (2014). Wild Sex in Zebrafish: Loss of the Natural Sex Determinant in Domesticated Strains, Genetics, 198 (3) 1291-1308. DOI: 10.1534/genetics.114.169284 http://www.genetics.org/content/198/3/1291.full
GENOME & SYSTEMS BIOLOGY
Reed L.K., K. Lee, Z. Zhang, L. Rashid, A. Poe, B. Hsieh, N. Deighton, N. Glassbrook, R. Bodmer & G. Gibson (2014). Systems Genomics of Metabolic Phenotypes in Wild-Type Drosophila melanogaster, Genetics, 197 (2) 781-793. DOI: 10.1534/genetics.114.163857 http://www.genetics.org/content/197/2/781.full
GENOME INTEGRITY & TRANSMISSION
Bauerly E., S. E. Hughes, D. R. Vietti, D. E. Miller, W. McDowell & R. S. Hawley (2014). Discovery of Supernumerary B Chromosomes in Drosophila melanogaster, Genetics, 196 (4) 1007-1016. DOI: 10.1534/genetics.113.160556 http://www.genetics.org/content/196/4/1007.full
Vrooman L.A., S.I. Nagaoka, T. J. Hassold & P. A. Hunt (2014). Evidence for Paternal Age-Related Alterations in Meiotic Chromosome Dynamics in the Mouse, Genetics, 196 (2) 385-396. DOI: 10.1534/genetics.113.158782 http://www.genetics.org/content/196/2/385.full
METHODS, TECHNOLOGY, & RESOURCES
Raj A., M. Stephens & J. K. Pritchard (2014). fastSTRUCTURE: Variational Inference of Population Structure in Large SNP Data Sets, Genetics, 197 (2) 573-589. DOI: 10.1534/genetics.114.164350 http://www.genetics.org/content/197/2/573.full
PERSPECTIVES
Wilkins A.S., R.W. Wrangham & W. T. Fitch (2014). The “Domestication Syndrome” in Mammals: A Unified Explanation Based on Neural Crest Cell Behavior and Genetics, Genetics, 197 (3) 795-808. DOI: 10.1534/genetics.114.165423 http://www.genetics.org/content/197/3/795.full
POPULATION & EVOLUTIONARY GENETICS
de Vladar H.P. & N. Barton (2014). Stability and Response of Polygenic Traits to Stabilizing Selection and Mutation, Genetics, 197 (2) 749-767. DOI: 10.1534/genetics.113.159111 http://www.genetics.org/content/197/2/749.full
Frenkel E.M., B.H. Good & M. M. Desai (2014). The Fates of Mutant Lineages and the Distribution of Fitness Effects of Beneficial Mutations in Laboratory Budding Yeast Populations, Genetics, 196 (4) 1217-1226. DOI: 10.1534/genetics.113.160069 http://www.genetics.org/content/196/4/1217.full
PRIMERS
Duina A.A., M.E. Miller & J. B. Keeney (2014). Budding Yeast for Budding Geneticists: A Primer on the Saccharomyces cerevisiae Model System, Genetics, 197 (1) 33-48. DOI: 10.1534/genetics.114.163188 http://www.genetics.org/content/197/1/33.full
STATISTICAL GENETICS & GENOMICS
Hormozdiari F., E. Kostem, E. Y. Kang, B. Pasaniuc & E. Eskin (2014). Identifying Causal Variants at Loci with Multiple Signals of Association, Genetics, 198 (2) 497-508. DOI: 10.1534/genetics.114.167908 http://www.genetics.org/content/198/2/497.full
METHODS, TECHNOLOGY, & RESOURCES: CRISPR
Gratz S.J., F.P. Ukken, C. D. Rubinstein, G. Thiede, L. K. Donohue, A. M. Cummings & K. M. O’Connor-Giles (2014). Highly Specific and Efficient CRISPR/Cas9-Catalyzed Homology-Directed Repair in Drosophila, Genetics, 196 (4) 961-971. DOI: 10.1534/genetics.113.160713 http://www.genetics.org/content/196/4/961.full
Li M., H. Yang, J. Zhao, L. Fang, H. Shi, M. Li, Y. Sun, X. Zhang, D. Jiang, L. Zhou & D. Wang & (2014). Efficient and Heritable Gene Targeting in Tilapia by CRISPR/Cas9, Genetics, 197 (2) 591-599. DOI: 10.1534/genetics.114.163667 http://www.genetics.org/content/197/2/591.full
Kim H., T. Ishidate, K. S. Ghanta, M. Seth, D. Conte, M. Shirayama & C. C. Mello (2014). A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans, Genetics, 197 (4) 1069-1080. DOI: 10.1534/genetics.114.166389 http://www.genetics.org/content/197/4/1069.full
Arribere J.A., R.T. Bell, B. X. H. Fu, K. L. Artiles, P. S. Hartman & A. Z. Fire (2014). Efficient Marker-Free Recovery of Custom Genetic Modifications with CRISPR/Cas9 in Caenorhabditis elegans, Genetics, 198 (3) 837-846. DOI: 10.1534/genetics.114.169730 http://www.genetics.org/content/198/3/837.full
Paix A., Y. Wang, H. E. Smith, C.-Y. S. Lee, D. Calidas, T. Lu, J. Smith, H. Schmidt, M. W. Krause & G. Seydoux (2014). Scalable and Versatile Genome Editing Using Linear DNAs with Microhomology to Cas9 Sites in Caenorhabditis elegans, Genetics, 198 (4) 1347-1356. DOI: 10.1534/genetics.114.170423 http://www.genetics.org/content/198/4/1347.full
SPOTLIGHT IMAGES
Zimin A., K.A. Stevens, M. W. Crepeau, A. Holtz-Morris, M. Koriabine, G. Marcais, D. Puiu, M. Roberts, J. L. Wegrzyn, P. J. de Jong, D. B. Neale, S.L. Salzberg, J.A. Yorke & C.H. Langley (2014). Sequencing and Assembly of the 22-Gb Loblolly Pine Genome, Genetics, 196 (3) 875-890. DOI: 10.1534/genetics.113.159715 http://www.genetics.org/content/196/3/875.full
Wegrzyn J.L., J.D. Liechty, K. A. Stevens, L.-S. Wu, C. A. Loopstra, H. A. Vasquez-Gross, W. M. Dougherty, B. Y. Lin, J. J. Zieve, P. J. Martínez-García, C. Holt, M. Yandell, A.V. Zimin, J.A. Yorke, M.W. Crepeau, D. Puiu, S.L. Salzberg, P.J. de Jong, K. Mockaitis, D. Main, C.H. Langley & D.B. Neale (2014). Unique Features of the Loblolly Pine (Pinus taeda L.) Megagenome Revealed Through Sequence Annotation, Genetics, 196 (3) 891-909. DOI: 10.1534/genetics.113.159996 http://www.genetics.org/content/196/3/891.full
Stronach B., A.L. Lennox & R. A. Garlena (2014). Domain Specificity of MAP3K Family Members, MLK and Tak1, for JNK Signaling in Drosophila, Genetics, 197 (2) 497-513. DOI: 10.1534/genetics.113.160937 http://www.genetics.org/content/197/2/497.full
Wilkening S., G. Lin, E. S. Fritsch, M. M. Tekkedil, S. Anders, R. Kuehn, M. Nguyen, R. S. Aiyar, M. Proctor, N. A. Sakhanenko, D. J. Galas, J. Gagneur, A. Deutschbauer & L.M. Steinmetz (2014). An Evaluation of High-Throughput Approaches to QTL Mapping in Saccharomyces cerevisiae, Genetics, 196 (3) 853-865. DOI: 10.1534/genetics.113.160291 http://www.genetics.org/content/196/3/853.full
Bannister S., O. Antonova, A. Polo, C. Lohs, N. Hallay, A. Valinciute, F. Raible & K. Tessmar-Raible (2014). TALENs Mediate Efficient and Heritable Mutation of Endogenous Genes in the Marine Annelid Platynereis dumerilii, Genetics, 197 (1) 77-89. DOI: 10.1534/genetics.113.161091 http://www.genetics.org/content/197/1/77.full
Zantke J., S. Bannister, V. B. V. Rajan, F. Raible & K. Tessmar-Raible (2014). Genetic and Genomic Tools for the Marine Annelid Platynereis dumerilii, Genetics, 197 (1) 19-31. DOI: 10.1534/genetics.112.148254 http://www.genetics.org/content/197/1/19.full
LaFave M.C., G.K. Varshney, M. Vemulapalli, J. C. Mullikin & S. M. Burgess (2014). A Defined Zebrafish Line for High-Throughput Genetics and Genomics: NHGRI-1, Genetics, 198 (1) 167-170. DOI: 10.1534/genetics.114.166769 http://www.genetics.org/content/198/1/167.full
Brooks S.S., A.L. Wall, C. Golzio, D. W. Reid, A. Kondyles, J. R. Willer, C. Botti, C. V. Nicchitta, N. Katsanis & E. E. Davis (2014). A Novel Ribosomopathy Caused by Dysfunction of RPL10 Disrupts Neurodevelopment and Causes X-Linked Microcephaly in Humans, Genetics, 198 (2) 723-733. DOI: 10.1534/genetics.114.168211 http://www.genetics.org/content/198/2/723.full
G3 SPOTLIGHT CITATIONS:
INVESTIGATIONS
Burton J.N., I. Liachko, M. J. Dunham & J. Shendure (2014). Species-Level Deconvolution of Metagenome Assemblies with Hi-C-Based Contact Probability Maps, G3, 4 (7) 1339-1346. DOI: 10.1534/g3.114.011825 http://www.g3journal.org/content/4/7/1339.full
Atencio D., C. Barnes, T. M. Duncan, I. M. Willis & S. D. Hanes (2014). The Yeast Ess1 Prolyl Isomerase Controls Swi6 and Whi5 Nuclear Localization, G3, 4 (3) 523-537. DOI: 10.1534/g3.113.008763 http://www.g3journal.org/content/4/3/523.full
Mezmouk S. & J. Ross-Ibarra (2014). The Pattern and Distribution of Deleterious Mutations in Maize, G3, 4 (1) 163-171. DOI: 10.1534/g3.113.008870 http://www.g3journal.org/content/4/1/163.full
Spivakov M., T.O. Auer, R. Peravali, I. Dunham, D. Dolle, A. Fujiyama, A. Toyoda, T. Aizu, Y. Minakuchi, F. Loosli & K. Naruse, E. Birney & J. Wittbrodt (2014). Genomic and Phenotypic Characterization of a Wild Medaka Population: Towards the Establishment of an Isogenic Population Genetic Resource in Fish, G3, 4 (3) 433-445. DOI: 10.1534/g3.113.008722 http://www.g3journal.org/content/4/3/433.full
Glater E.E., M.V. Rockman & C. I. Bargmann (2014). Multigenic Natural Variation Underlies Caenorhabditis elegans Olfactory Preference for the Bacterial Pathogen Serratia marcescens, G3, 4 (2) 265-276. DOI: 10.1534/g3.113.008649 http://www.g3journal.org/content/4/2/265.full
Hahn M.W., S.V. Zhang & L. C. Moyle (2014). Sequencing, Assembling, and Correcting Draft Genomes Using Recombinant Populations, G3, 4 (4) 669-679. DOI: 10.1534/g3.114.010264 http://www.g3journal.org/content/4/4/669.full
Rajagopal N., J. Ernst, P. Ray, J. Wu, M. Zhang, M. Kellis & B. Ren (2014). Distinct and Predictive Histone Lysine Acetylation Patterns at Promoters, Enhancers, and Gene Bodies, G3, 4 (11) 2051-2063. DOI: 10.1534/g3.114.013565 http://www.g3journal.org/content/4/11/2051.full
Engel S.R., F.S. Dietrich, D. G. Fisk, G. Binkley, R. Balakrishnan, M. C. Costanzo, S. S. Dwight, B. C. Hitz, K. Karra, R. S. Nash, S. Weng, E.D. Wong, P. Lloyd, M.S. Skrzypek, S.R. Miyasato, M. Simison & J.M. Cherry (2014). The Reference Genome Sequence of Saccharomyces cerevisiae: Then and Now, G3, 4 (3) 389-398. DOI: 10.1534/g3.113.008995 http://www.g3journal.org/content/4/3/389.full
Rice W.R. (2014). An X-Linked Sex Ratio Distorter in Drosophila simulans That Kills or Incapacitates Both Noncarrier Sperm and Sons, G3, 4 (10) 1837-1848. DOI: 10.1534/g3.114.013292 http://www.g3journal.org/content/4/10/1837.full
Rogers R.L., L. Shao, J. S. Sanjak, P. Andolfatto & K. R. Thornton (2014). Revised Annotations, Sex-Biased Expression, and Lineage-Specific Genes in the Drosophila melanogaster Group, G3, 4 (12) 2345-2351. DOI: 10.1534/g3.114.013532 http://www.g3journal.org/content/4/12/2345.full
Pang A.W.C., J.R. MacDonald, R. K. C. Yuen, V. M. Hayes & S. W. Scherer (2014). Performance of High-Throughput Sequencing for the Discovery of Genetic Variation Across the Complete Size Spectrum, G3, 4 (1) 63-65. DOI: 10.1534/g3.113.008797 http://www.g3journal.org/content/4/1/63.full
Vaughn J.N., R.L. Nelson, Q. Song, P. B. Cregan & Z. Li (2014). The Genetic Architecture of Seed Composition in Soybean Is Refined by Genome-Wide Association Scans Across Multiple Populations, G3, 4 (11) 2283-2294. DOI: 10.1534/g3.114.013433 http://www.g3journal.org/content/4/11/2283.full
Walther A., A. Hesselbart & J. Wendland (2014). Genome Sequence of Saccharomyces carlsbergensis, the World’s First Pure Culture Lager Yeast, G3, 4 (5) 783-793. DOI: 10.1534/g3.113.010090 http://www.g3journal.org/content/4/5/783.full
MUTANT SCREEN REPORT
Hayashi R., S.M. Wainwright, S. J. Liddell, S. M. Pinchin, S. Horswell & D. Ish-Horowicz (2014). A Genetic Screen Based on in Vivo RNA Imaging Reveals Centrosome-Independent Mechanisms for Localizing gurken Transcripts in Drosophila, G3, 4 (4) 749-760. DOI: 10.1534/g3.114.010462 http://www.g3journal.org/content/4/4/749.full
SPOTLIGHT IMAGES
Lehnert E.M., M.E. Mouchka, M. S. Burriesci, N. D. Gallo, J. A. Schwarz & J. R. Pringle (2014). Extensive Differences in Gene Expression Between Symbiotic and Aposymbiotic Cnidarians, G3, 4 (2) 277-295. DOI: 10.1534/g3.113.009084 http://www.g3journal.org/content/4/2/277.full
Jovelin R., J.S. Comstock, A. D. Cutter & P. C. Phillips (2014). A Recent Global Selective Sweep on the age-1 Phosphatidylinositol 3-OH Kinase Regulator of the Insulin-Like Signaling Pathway Within Caenorhabditis remanei, G3, 4 (6) 1123-1133. DOI: 10.1534/g3.114.010629 http://www.g3journal.org/content/4/6/1123.full
Sikkink K.L., R.M. Reynolds, C. M. Ituarte, W. A. Cresko & P. C. Phillips (2014). Rapid Evolution of Phenotypic Plasticity and Shifting Thresholds of Genetic Assimilation in the Nematode Caenorhabditis remanei, G3, 4 (6) 1103-1112. DOI: 10.1534/g3.114.010553 http://www.g3journal.org/content/4/6/1103.full
INVESTIGATIONS: CRISPR
Xue Z., M. Ren, M. Wu, J. Dai, Y. S. Rong & G. Gao (2014). Efficient Gene Knock-out and Knock-in with Transgenic Cas9 in Drosophila, G3, 4 (5) 925-929. DOI: 10.1534/g3.114.010496 http://www.g3journal.org/content/4/5/925.full
Lee J.S., S.-J. Kwak, J. Kim, A.-K. Kim, H. M. Noh, J.-S. Kim & K. Yu (2014). RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein, G3, 4 (7) 1291-1295. DOI: 10.1534/g3.114.012179 http://www.g3journal.org/content/4/7/1291.full
Ren X., Z. Yang, D. Mao, Z. Chang, H.-H. Qiao, X. Wang, J. Sun, Q. Hu, Y. Cui, L.-P. Liu, J.-Y. Ji, J. Xu & J.-Q Ni (2014). Performance of the Cas9 Nickase System in Drosophila melanogaster, G3, 4 (10) 1955-1962. DOI: 10.1534/g3.114.013821 http://www.g3journal.org/content/4/10/1955.full
Xue Z., M. Wu, K. Wen, M. Ren, L. Long, X. Zhang & G. Gao (2014). CRISPR/Cas9 Mediates Efficient Conditional Mutagenesis in Drosophila, G3, 4 (11) 2167-2173. DOI: 10.1534/g3.114.014159 http://www.g3journal.org/content/4/11/2167.full
Gokcezade J., G. Sienski & P. Duchek (2014). Efficient CRISPR/Cas9 Plasmids for Rapid and Versatile Genome Editing in Drosophila, G3, 4 (11) 2279-2282. DOI: 10.1534/g3.114.014126 http://www.g3journal.org/content/4/11/2279.full
Upadhyay S.K., J. Kumar, A. Alok & R. Tuli (2014). RNA-Guided Genome Editing for Target Gene Mutations in Wheat, G3, 3 (12) 2233-2238. DOI: 10.1534/g3.113.008847 http://www.g3journal.org/content/3/12/2233.full
Vilain S., R. Vanhauwaert, Maes, N. Schoovaerts, L. Zhou, S. Soukup, R. da Cunha, E. Lauwers, M. Fiers & P. Verstreken (2014). Fast and Efficient Drosophila melanogaster Gene Knock-Ins Using MiMIC Transposons, G3, 4 (12) 2381-2387. DOI: 10.1534/g3.114.014803 http://www.g3journal.org/content/4/12/2381.full
Zhang X., W.H. Koolhaas & F. Schnorrer (2014). A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila, G3, 4 (12) 2409-2418. DOI: 10.1534/g3.114.013979 http://www.g3journal.org/content/4/12/2409.full