The figure illustrates the use of the reagents described in Dionne et al. (GENETICS 2018) to generate cell-type-specific GAL4 lines using the split-Gal4 method. The center three brains in the center column show split-GAL4 lines for cell types involved in the circadian clock (large and small LNv cells) derived from the same AD hemidriver line (partial brain of the generation 1 GAL4 pattern shown on the left in red) combined with 3 different DBD hemidriver lines (shown in different colors on the right). Image by Heather Dionne / Janelia Flylight Project Team. See Dionne et al.

The Annual Drosophila Research Conference is the premier place to share your research with the fruit fly community and discover cutting-edge advances. Presentations cover the full diversity of Drosophila investigations, from genetics to molecular biology, cell biology, development, immunology, physiology, neuroscience, evolution, and more.

Now in its 60th year, the Drosophila conference is meeting in Dallas, Texas, from March 27–31, 2019, and will host an exciting new plenary — the Techniques & Technology session. On Saturday, March 30 from 7:30 p.m.–9:30 p.m., join your colleagues at the Sheraton Dallas to hear fascinating presentations from eight researchers leading the way in advanced biomedical methods and discussing valuable resources:


7:30  Gerald RubinNew Tools and Methods for Neuronal Circuit Analysis in Drosophila

7:45  Yulong LiSpying on the dynamics of acetylcholine, dopamine, octopamine, and 5-HT in fly’s brain by constructing new genetically-encoded GRAB sensors

8:00  Nick MatinyanSelectable, drug-based genetics and transgenesis in Drosophila melanogaster

8:15  Leslie Ann KoyamaBellymount: A novel method for longitudinal, intravital imaging of abdominal organs in adult Drosophila

8:30  Giorgio GilestroGAL4s, LEGOs, and 3D-printers: the genetic toolbox of the 21st-century Drosophilist

8:45  Stein AertsTechniques and computational methods for single-cell regulatory genomics in Drosophila

9:00  Steven MarygoldFlyBase updates

9:15  Oguz KancaA Gene Disruption Project (GDP) update: using CRISPR with PCR-generated homology donors to knock-in Swappable Integration Cassettes in introns of genes in flies and in S2 cells

Session Chairs: Hugo Bellen, Lena Riabinina, and Julie Simpson


Register for Dros19 today to make sure you don’t miss the Technology & Techniques plenary session, and follow @GeneticsGSA on Twitter for more information on the 2019 fly meeting!

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