Articles tagged Sequencing
(23 results)

February marks the launch of a crisp new look and improved navigation at the G3 website. Go check it out; we’re very proud of the design! We are also unveiling a new cover layout that allows the art submitted by our authors to shine. This month’s cover celebrates the first published genome assembly of the Canadian…

Towering sugar pine trees dominate the mountain forests of California and Oregon. They are the tallest pine trees in the world, regularly growing to skyscraper heights of over 100 meters. But these forest behemoths are under attack from a very tiny foe: an invasive fungus. White pine blister rust was accidentally introduced to western North…

When it comes to genotyping technology, poop genetics is stuck in the 1990s. While most geneticists are now awash in genome-scale data from thousands of individuals, those who depend on  fecal and other non-invasively collected samples still rely on old-school, boutique panels of a dozen or so genetic markers. But feces — along with fur,…

The dizzying array of wing patterns in Heliconius butterflies has served as a model for evolution and adaptation in the wild for more than a century. The genus is most famous for the way different species within a geographic region tend to converge on similar wing markings—known as biological mimicry. In the latest issue of…

A fisherman trying to catch rainbow trout (Oncorhynchus mykiss) needs the right tools: proper flies, a strong rod, and a little bit of know-how. A scientist trying to understand the genetic population structure of rainbow trout in the Fall River watershed of northern California also relies on a trusty toolkit – albeit a very different…

Short-read sequencing has fueled the acceleration of genetic research But though these next-generation methods are fast and efficient, they can’t do everything well. One important area in which short-reads fall short is detecting structural variants (SV), where chunks of the genome are deleted, inserted, repeated, inverted, or in some other way shuffled around compared to…

If you’ve ever watched a procedural crime-solving show on television, you’re sure to have seen a lab tech magically produce results from a complicated assay in mere minutes. If you’re a wet lab scientist, you’ve probably found yourself wishing that “CSI technology” were real so you didn’t have to spend your whole day running PCRs…

Over the last decade, advances in next-generation sequencing technology have given rise to many findings increasing our understanding of human disease and natural variation within species. Sequencing of the exome, the small fraction of the genome encompassing all exons of protein coding genes, has gained popularity as an inexpensive alternative to sequencing the entire genome.…

Primary ciliary dyskinesia (PCD) is a disorder characterized by chronic respiratory distress that has a history of going undiagnosed. Combined whole-exome sequencing (WES) and copy-number variant (CNV) analysis can aid in diagnosing patients with PCD, according to research published in G3 this month. PCD arises from mutations in cilia-associated genes—and results from the paralysis of…

For Lake Whitefish, history has repeated itself. Across the St. John River region that spans Québec and Maine, these freshwater fish have continually evolved in the same way. Within the many individual lakes in this area, Lake Whitefish have diverged into two groups differentiated by size and body shape. These two groups, known as “dwarf”…

The most diverse of all human genes encode a set of proteins at the frontline of our immune system. Many different Human Leukocyte Antigen (HLA) proteins are encoded by genes clumped together in one portion of the human genome known as the major histocompatibility complex region. HLA proteins sit on the surface of cells and…